SLDAssay IUPM Calculator v1.0

This web tool calculates the maximum likelihood estimate of concentration of infectious units from a single serial limiting dilution (SLD) assay. This web tool also calculates corresponding exact and asymptotic confidence intervals, and a goodness-of-fit p-value. While this package was developed with the purpose of estimating IUPM, it is applicable to SLD assays in general.

Input Table

In the table below, each row corresponds to a dilution level. Please input the number of cells per well (Col 1), number of replicates per dilution level (Col 2), and number of positive wells per dilution level (Col 3) in the table below. The number of dilution levels may be controlled by pressing the +/- buttons.
Well Size # of Replicates # Positive
1000000
2
200000
2
40000
2
8000
2
1600
2
320
2
When Monte Carlo sampling is necessary, it is recommended to set the number of Monte Carlo samples as large as computationally feasible. Default is no MC sampling, unless more than 15,000 possible positive well outcomes exist, in which case 15,000 MC samples are taken. Use 0 MC samples for exact computation.

Results


              
Exact results are recommended for use in practice.
              

Documentation

References:
Click Here to View Article

Acknowledgements

Contact Information


SLDAssay IUPM Calculator v1.0 was created and updated by Andrew G. Allmon on 17 October 2020 .